The south-north relay mode of abalone culture, a common practice in China, was designed to increase the survival rate of abalone in the south in summer. The abalone was usually transported to the north(Rongcheng, Shandong) in April from southern China(Lianjiang, Fujian)and returned to the south in November. In this study, the impact of this mode on the nutritive compositions of abalone (Haliotis discus hannai) was investigated. The main method was the series of GB.5009. The determined indexes include moisture, cholesterol, ash, protein, lipid, glycogen, collagen, mineral elements(Mg, Al, Ca, Mn, Fe, Cu, Zn, Se),delicious amino acids(aspartic acid, glutamic acid, glycine, alanine, arginine, methionine, lysine),taurine and fatty acid. SPSS 24.0 statistical software(independent sample t-test)was used to determine the significance of the differences between two groups. The experimental results were expressed as mean ±standard deviation.Two groups of adult abalone(two years old)with the same algae diet (Gracilaria lemaneiformis) were sampled for the analysis in December, 2017. One group was cultured in Lianjiang, Fujian (Southern China) without migration (LJ-DD) and the other was moved to Shandong (Northern China) before the summer and returned to Lianjiang in November (SD-DD). Live samples were moved to laboratory within 24 hours, taking abalone foot muscle to freeze drying after dissection and grinding into powder. These powder were saved in −80 ℃ freezer. The results showed that contents of ash and collagen were higher in LJ-DD, but not significant. The cholesterol, lipid and glycogen content were higher in SD-DD, but not significant. Moisture content was significantly higher in SD-DD (76.50%WW) than in LJ-DD(73.70%WW) while total protein content was significantly lower in SD-DD(48.40%DW). For mineral profile, Se content was significantly higher in LJ-DD(0.07 mg/100g)than SD-DD(0.05 mg/100g). Besides, Mg and Al contents were higher in SD-DD and Ca, Fe, Cu and Zn contents were lower in SD-DD, but the differences were not significant. For amino acid profile, the contents of glutamic acid, arginine, lysine and total delicious amino acid were significantly higher in LJ-DD than in SD-DD. Besides, taurine content was significantly lower in SD-DD than in LJ-DD. For fatty acid profile, C20:3n6 content was significantly higher in LJ-DD than in SD-DD. SFA and N-3 fatty acid were higher in SD-DD，and MUFA, PUFA and EPA+DHA contents were higher in LJ-DD，but not significantly. The value of N6/N3 was lower in SD-DD than in LJ-DD, but the differences were not significant. Overall，two groups had similar composition, but SD-DD had higher nutritional values than LJ-DD. To summarize, the south-north relay mode has both some negative influences and some positive influences on the nutritional quality of H. discus hannai, but such difference is not significant as a whole.