The purpose of this study is to find out the effects of compound Chinese herbs (Radix acutellariae, Puerarin, Coptidis, rhizome, Honeysuckle, Isatidis radix, Astragalus radix, Glycyrrhiza wralensis Fisch, Bupleurum, Angelica sinensis, Hawthorn, citrusa and Poria cocos) on growth digestive enzyme, immunologic factors and WSSV-resistant capacity of Litopenaeus vannamei and provide scientific basis for the healthy culture of it. Litopenaeus vannameis (0.026±0.007) g were randomly divided into 6 groups with 3 relicates per group and 160 shrimps per replicate. The shrimps were fed 28-days with basal diets supplemented with 0 (control group), 0.4%, 0.8%, 1.2%, 1.6%, 2.0% of compound Chinese herbs, respectively. The results showed that the growth performance (WGR、SGR、FR and SR) in experimental groups was significantly increased compared with that of the control group, and the 0.8% and 1.2% supplemental groups showed the best results. While the FCR was significantly decreased. The high activities of Digestive enzyme (Amylase, Potease and Lipase) and immunologic factors (SOD, GPT and GOT) on hepatopancreas of Litopenaeus vannamei were found in high supplemental groups, while with the time went by they were found in little supplemental groups, and the 0.8% and 1.2% supplemental groups showed the best results. Compared with control group, the content of MDA in 0.4%, 0.8% and 1.2% supplemental groups were significantly decreased. The shrimp in the control group and 0.4% supplemental groups were 100% death after infected with WSSV, however the time of the first death shrimp, the time of 50% death and the time of 100% death in 0.4% supplemental groups were delayed by 8, 16 and 8 h compared with that of the control group, respectively. The survival rate of 0.8% supplemental group was 33.3%, significantly higher than that of other groups. In short, compound Chinese herbs can promote growth, digestion, immune and WSSV-resistant ability, and 0.8% and 1.2% supplemental groups indicated best results.