The paper studied the effects of dry storage and wet storage on biochemical metabolism of live scallop Patinopecten yessoensis during post-harvest process, and discussed the influences of stressors (exposure, mechanical shock and temperature). Dry storage is stored with ice for 4 days. Wet storage is stored with circulating seawater, including three groups:5℃, 10℃ and 10℃ with mechanical shock, for 7 days. We analyzed the biochemical index of ATP related compounds, glycogen, AEC value, pH value and water soluble protein. The results showed that under dry storage, the ATP content, AEC and pH value in the adductor muscle of scallop decreased rapidly, eventually leading death. Under wet storage of 5 and 10℃, the scallop kept higher ATP content, AEC and pH value, and wet storage of 5℃ could maintain higher glycogen content than 10℃. Also at 10℃, adding mechanical shock will lead to the decrease of ATP content, AEC value and the increase of the consumption of glycogen. Therefore, the biochemical metabolism of live scallops is closely related to the post-harvest handling. Exposure is the most harmful stressor, the mechanical shock and temperature could also affect the physiological status.