The order Decapoda, with most species and high economic value in crustaceans, was divided to two sub-orders:Dendrobranchiata (Penaeidea etc.) and Pleocyemata (Caridea, Astacidea, Palinura, and Brachyura etc.). The full length of vitellogenin cDNA in Decapoda is about 8 kilobases (kb) in size, and encoded 2500-2600 amino acid residues. Vg gene organization and expression pattern in Decapods is highly conserved. But the site and model of vitellogenesis is obviously different. In the artificial control conditions, there is a distinguished different exhibition between the Penaeidea shrimps and Caridea or Brachyura, etc.. The ovary of Penaeid shrimps is difficult to mature, and usually people have to remove their eye-stalk to promote the gonad (ovary) development, however, the species in Pleocyemata like Caridea or Brachyura are very easy to mature, even people need to inhibit their ovary to develop early with control temperature and nutrition. Vg in Penaeidea shrimps is synthesized in ovary and hepatopancreas coordinately, and ovary contributes more than hepatopancreas usually, especially during the early stage of the gonadal development. So penaeid shrimps may constitute a unique model for vitellogenesis, showing intra-ovarian gene expression and synthesis of yolk protein. However, in Pleocyemata such as Caredea, Brachyura etc., the ovary also joins the vitellogenesis, but the hepatopancreas plays the major role and its contribution rate accounts for more than 90%. Why so big difference of vitellogenesis mechanism and gonad maturation in captive condition exists in these two groups of crustacean is discussed in this paper.