Quorum sensing(QS)is a cell-cell communication system with population density.QS enables bacteria to synchronize specific gene expression,such as luminescence,virulence,motility,sporulation and biofilm formation.QS inhibitory effect can disrupt signal molecules of pathogens,thereby reducing its virulence factor expression,has broad new opportunities for therapeutic or environmental application.However,very little is known about the animal safety and ecology environment evaluation of the bacteria with QS inhibition effect.The purpose of the present study was to investigate the animal safety and ecology evaluation of Bacillus licheniformis T-1 with QS inhibition effect isolated by ourselves and provide a scientific basis for its use in the future.According to the national standard(GB)acute toxicity methods,the animal safety test and ecology environment evaluation of the Bacillus licheniformis T-1 was conducted.We use crucian carp,zebrafish and mice as test animal for safety evaluation,and use Chlorella vulgaris,Daphnia magna and Brachionus calyciflorus as test objects for ecological environment evaluation.The results showed that the crucian carp were intraperitoneally injected with the concentration of 11 200 mg/L(2.6×10¹¹ CFU/mL),zebrafish were bathed in the concentration of 2 240 mg/L(5.2×10¹⁰ CFU/mL)and SPF mice(ICR)were gavaged with the 16 800 mg/L(3.9×10¹¹ CFU/mL)concentration of B.licheniformis T-1 were all healthy and without any diseased symptoms or dead in continuous observation 96 h.Different concentration of B.licheniformis T-1 was non-toxic on Chlorella vulgaris,whereas promoted them to grow.B.licheniformisT-1 was nontoxic to the growth of Daphnia magna in continuous observation 24h.B.licheniformis T-1 was nontoxic to the Brachionus calyciflorus growth in 24 h and could promote their growth in high concentration.To conclude,the results in the present experiments indicated that the B.licheniformis T-1 with the inhibitory effect of quorum sensing was nontoxic on crucian carp,zebrafish and mice,and had no adverse effect on plankton in water ecological environment.It has the potential for probiotics in the future.