To provide information for controlling bacterial disease of aquatic animals,this study reveals the diversity and resistance of the drug-resistant bacteria around Anguilla rostrata and the farming ponds.Drug-resistant bacteria in the samples from intestine,epidermis,gill of A.rostrata and the pond water were screened by five antimicrobials.Their antibiotic resistance was determined by the Kirby-Bauer disc diffusion method and taxonomic status was identified by phylogenetic analysis of 16S rDNA sequences.The results showed that 108 resistant strains were isolated and classified into 20 genera,such as Aeromonas,Citrobacter,and Acinetobacter.The percentage of multiple resistance to three or more varieties of antibiotics was 93.5%,while the rate judged by three or more classes of antibiotics was 86.1%.The frequency of resistance to amoxicillin(90.7%)was high,and so with tetracycline,rifampicin,sulfonamides,and amphenicols(60%-80%).On the contrary,resistance to cefotaxime,neomycin and quinolones(less than 20%)was unfrequent.The multiple antibiotic resistance(MAR)indexes of isolates from intestine,epidermis,gill,and water were 0.40,0.41,0.42 and 0.47 respectively,which showed the high risk of MAR in every ecological sample,especially in the water sample.Among genera,MAR indexes of Citrobacter(0.58)and Klebsiella(0.61)were high,while that of Acinetobacter(0.21)was low relatively.Multi-drug resistant bacteria turned out to be ubiquitous in A.rostrata and the farming water,to which enough attention should be paid.Resistant bacteria from A.rostrata and water environments were mostly sensitive to some fishery drugs such as norfloxacin and neomycin.So they could be preferential drugs to treat the bacterial disease in aquaculture.