延迟首次投喂对胭脂鱼仔鱼氨基酸和脂肪酸的影响
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西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,重庆水产科学研究所,重庆师范大学进化生理与行为学实验室重庆市动物生物学重点实验室,西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室

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公益性行业(农业)科研专项(201203086)


Effects of different delayed first feeding time on fatty acid and amino acids of Myxocyprinus asiaticus larvae
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Key Laboratory of freshwater Fish Reproduction and Development Ministry of Education,School of Life Science Southwest University,Key Laboratory of freshwater Fish Reproduction and Development Ministry of Education,School of Life Science Southwest University,Key Laboratory of freshwater Fish Reproduction and Development Ministry of Education,School of Life Science Southwest University,Key Laboratory of freshwater Fish Reproduction and Development Ministry of Education,School of Life Science Southwest University,Key Laboratory of freshwater Fish Reproduction and Development Ministry of Education,School of Life Science Southwest University,Key Laboratory of freshwater Fish Reproduction and Development Ministry of Education,School of Life Science Southwest University,Key Laboratory of freshwater Fish Reproduction and Development Ministry of Education,School of Life Science Southwest University,Key Laboratory of freshwater Fish Reproduction and Development Ministry of Education,School of Life Science Southwest University

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    摘要:

    为考察饥饿及恢复摄食对胭脂鱼仔鱼氨基酸和脂肪酸的影响.在水温(19±0.5)℃下,对胭脂鱼仔鱼实施延迟首次投喂0、1、3、5、7、9和11 d共7个处理,随后进行饱食投喂,分别在延迟处理结束时以及摄食后(19日龄和29日龄)取材对鱼体的含脂量、脂肪酸和氨基酸含量进行检测.结果如下:(1)随延迟首次投喂时间的增加,胭脂鱼仔鱼鱼体的脂肪含量呈显著下降趋势.在实验结束时(29日龄),各处理组仔鱼的脂肪含量均与对照组无显著性差异,表现出完全补偿效应.(2)胭脂鱼仔鱼在饥饿期间,主要以单不饱和脂肪酸作为能量代谢基质,按n-6>n-9>n-3顺序被先后利用,且C22∶6n-3(DHA)优先于C20∶5n-3(EPA)被保存下来.同时,鱼体中DHA和ARA的百分含量与仔鱼体质量和含水率存在极显著相关关系.(3)随延迟首次投喂时间的增加,胭脂鱼仔鱼的丙氨酸、异亮氨酸、亮氨酸和组氨酸显著下降;天冬氨酸和谷氨酸显著上升;而甘氨酸、赖氨酸和精氨酸则呈先上升后下降的趋势.结果表明:胭脂鱼仔鱼对饥饿有较强的适应性,在饥饿初期以消耗脂类物质为主,当脂类物质趋于阈值,氨基酸开始被大量消耗;胭脂鱼仔鱼在饥饿后恢复摄食时,鱼体氨基酸的恢复比脂肪酸更慢.

    Abstract:

    To investigate the effect of different delayed first feeding time on the amino acids and fatty acids of Myxocyprinus asiaticus larvae under the condition of water temperature(19±0.5)℃,we divided the larvae into 7 different initial delayed feeding time groups,1,3,5,7,9 and 11 delayed days denoted as group D0,D1,D3,D5,D7,D9 and D11 respectively,and subsequently fed at corresponding time,then detected the composition and content of the amino acids and fatty acid at different intervals,with total experimental period lasting 29 days.Results showed that,(1)The larvae body lipid content showed a significant downward trend when prolonging the starvation time.However,at the end of the experiment(29 days old),all of the other groups were not significantly different from the control group on lipid content,indicating a complete compensation effect.(2)During starvation,the monounsaturated fatty acids(MUFA)were utilized as energy substrates by M.asiaticus larvae,with the order of n-6>n-9>n-3 and C22∶6n-3(DHA)being preserved prior to C20∶5n-3(EPA).In addition,the contents(percentage)of DHA and ARA were significant different from the body weight and moisture content.(3)With increasing time of starvation,the alanine,isoleucine,leucine and histidine decreased significantly,while the aspartic acid and glutamic acid increased significantly,however,the glycine,lysine and arginine first increased and then decreased in M.asiaticus larvae.To sum up,first,M.asiaticus larvae have strong adaptability for starvation,and mainly consume lipids in the early starvation period,and then amino acids begin to be consumed when the lipids come near to the threshold.Then,it suggested a slower restoration of the amino acid than the fatty acids for M.asiaticus larvae after resuming feeding.

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王川,郭海燕,李秀明,袁建明,蒲德永,金丽,王志坚,张耀光.延迟首次投喂对胭脂鱼仔鱼氨基酸和脂肪酸的影响[J].水产学报,2015,39(1):75~87

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  • 收稿日期:2014-05-08
  • 最后修改日期:2014-10-09
  • 录用日期:2015-01-16
  • 在线发布日期: 2015-01-19
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