A 92-d feeding experiment was conducted to investigate the effects of dietary graded level of linseed oil(LO)on growth performance, fatty acids composition and lipid deposition of liver and muscle in juvenile turbot(Scophthalmus maximus)[mean initial body weight, (5.89±0.02)g].Four isonitrogenous and isoenergetic practical diets were formulated with LO replacing 0%, 33.3%, 66.7% and 100% fish oil(FO), respectively.Each diet was randomly fed to triplicate groups in indoor seawater recirculation system to apparent satiation.The results showed that survival rate(SR)and specific growth rate(SGR)of juvenile turbot were independent of dietary LO level(P>0.05).With increasing dietary LO level, feed intake(FI)of turbot significantly increased, while feed efficiency(FE)and apparent net protein utilization(ANPU)significantly decreased(P<0.05), and they were up to the maximum and minimum in 100% LO group, respectively.Apparent digestibility coefficient of dietary lipid, hepatosomatic index and lipid content of muscle were also independent of dietary LO level(P>0.05).Lipid content of liver in turbot significantly increased with increasing dietary LO levels(P<0.05), which in 100% LO group was significantly higher than that in 100%FO group(P<0.05).Fatty acid composition of total lipid in liver and muscle was closely correlated with those in diets.With increasing dietary LO levels, the content of linoleic acid and linolenic acid significantly increased, while the content of EPA and DHA significantly decreased(P<0.05).These results suggested that on basis of nutritional quality, the LO replacement level in turbot diet should be lower than 66.7%, and the content of n3 very long chain-polyunsaturated fatty acid in the turbot diet should be higher than 0.8%.