The transcriptome of the Huso dauricus' muscle tissue was sequenced by a new generation of high throughput sequencing technology in order to fill a gap which has hitherto existed in the research field of gene sequences of H.dauricus and to preserve the gene resources,at the same time to lay the ground for discovering functional genes and cloning new genes,etc.Obtaining 14 447 211 200 bp of the original data,it generated 55 531 Unigenes which ranged from 300 bp to 32 613 bp in length,the average length was 941 bp.Similar searches were made by Bioinformatics methods which was against the Nr database of these Unigenes,in addition,it made gene functional description of the GO and analysis of metabolic pathways of KEGG.The result indicated that 20 735(37.34%)of these Unigenes have significant matches.The unigenes GO functions in the transcriptome library were divided into 3 categories:biological process,cellular component and molecular function with 56 branches;it could be divided into 290 classes taking the KEGG database as a reference.