In order to determine the transport conditions of large yellow croaker,this paper studied changes of cortisol,glycogen and lactate of large yellow croaker juveniles under the conditions of different density and salinity during transportation.The average body mass of large yellow croaker juveniles was 0.12±0.03 g,three treatments of density(2,4 and 8 g/L)and five treatments of salinity(5,10,15,20 and 25),totally fifteen treatments were set up.After the transportation of 10 h at 16.5℃,mortality and total ammonia nitrogen in water of each group were determined.Results showed that groups with 4 and 8 g/L density(1.33%,2.63%)had significantly higher mortalities than the group of density of 2 g/L(0.77%)(P<0.05),and group of salinity 25(0.44%)had a significantly lower mortality than that at salinity of 10 and 15(1.94%,1.67%)(P<0.05),groups with salinity 10 and15 had a significantly lower mortality than salinity 5 group(2.72%)(P<0.05).Total ammonia nitrogen in water was significantly increased after transportation compared with before(0.15 mg/L)(P<0.05),the total ammonia nitrogen in water were significantly different among different density groups(P<0.05),total ammonia nitrogen content in high density group was higher than that in low density groups; 8 g/L density under the condition of salinity 5 group(1.63 mg/L)had a significantly higher content of total ammonia nitrogen in water salinity of 15(1.38 mg/L),20(1.34 mg/L)and 25(1.31 mg/L)salinity groups,4 g/L density under the condition of 5 salinity group(1.31 mg/L)had a significantly higher content of total ammonia nitrogen in water with the salinity group,2 g/L density under the condition of 5 group(0.83 mg/L)had a significantly higher content of total ammonia nitrogen in water salinity 25 group(0.49 mg/L)(P<0.05).Cortisol levels after transportation were significantly increased compared before transportation(6 476.35 ng/L)(P<0.05); however,cortisol levels showed no significant difference between groups(P>0.05).Glycogen content in each group was significantly reduced compared before transportation(4.37 mg/g prot)(P<0.05),and there was no significant difference between groups(P>0.05).Lactic acid content in each group was significantly higher than that before transportation(0.57 mmol/g prot)(P<0.05),the density had no significant effect on lactic acid levels(P>0.05),for salinity,lactic acid content of salinity 5 group at each density(1.91 mmol/g prot,2.02 mmol/g prot,1.76 mmol/g prot)was significantly higher than salinity 20(1.55 mmol/g prot,1.21 mmol/g prot,1.69 mmol/g prot)and 25 group(1.36 mmol/g prot,1.44 mmol/g prot,1.26 mmol/g prot)(P<0.05),lactic acid content of salinity 10 group(1.77 mmol/g prot,1.83 mmol/g prot,1.60 mmol/g prot)was significantly higher than salinity 25 group(1.36 mmol/g prot,1.44 mmol/g prot,1.26 mmol/g prot)(P<0.05).Statistical analysis showed high transport density and lower salinity can aggravate the stress responses of large yellow croaker juveniles,suggesting that in the conditions of transportation,and with specifications of large yellow croaker larvae around 0.1 g,the transportation requirements should not be higher than 8 g/L in density,and lower than 5 of salinity.