光照强度对三疣梭子蟹呼吸代谢的影响
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中国海洋大学教育部海水养殖重点实验室,中国海洋大学教育部海水养殖重点实验室,中国海洋大学教育部海水养殖重点实验室,中国海洋大学教育部海水养殖重点实验室

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“十二五”国家科技支撑计划(2011BAD13B03)


Effects of light intensity on the respiratory metabolism of swimming crab(Portunus trituberculatus)
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The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China,The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China,,

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    摘要:

    在实验室条件下,研究了光照强度(0,200,800,1 500,3 500 lx)对三疣梭子蟹耗氧率、排氨率、肌肉中乳酸含量、血淋巴能源物质及己糖激酶(HK)、丙酮酸激酶(PK)、琥珀酸脱氢酶(SDH)和乳酸脱氢酶(LDH)4种呼吸代谢酶活力的影响。结果发现,5种光照强度下,三疣梭子蟹的耗氧率和排氨率均有显著差异,200~1 500 lx下,蟹的耗氧率显著高于0和3 500 lx处理组(P<0.05);1 500 lx下,蟹的排氨率显著低于其他处理组(P<0.05);光照强度对三疣梭子蟹血淋巴中的总蛋白含量产生显著影响(P<0.05),但对氧氮比和血淋巴中的其他能源物质未产生显著影响(P>0.05);光照强度对三疣梭子蟹的肌肉乳酸含量产生显著影响(P<0.05),1 500 lx下,蟹肌肉乳酸含量显著低于其他处理组(P<0.05),说明该光照强度对蟹机体的胁迫小;1 500 lx光照下,蟹的HK和PK活力处在较高水平,表明机体糖酵解速率较高,产生更多的能量;SDH的活力处在较高水平,LDH处在较低水平,表明蟹的有氧代谢能力高,有利于蟹的持续运动。研究表明,1 500 lx光照下,三疣梭子蟹机体受到的胁迫小,整体代谢水平较高,是工厂化养殖较为理想的光照参数。

    Abstract:

    Indoor experiments were conducted to investigate the effect of constant light intensity on physiology and ecology characteristics of swimming crab(Portunus trituberculatus).The oxygen consumption rate(OCR),ammonia excretion rate(AER),O:N ratio,lactic acid(LD),energetic contents in hemolymph and respiratory enzyme activities including hexokinase(HK),pyruvate kinase(PK),succinate dehydrogenase(SDH)and lactate dehydrogenase(LDH)were tested in five constant light intensity treatments(0,200,800,1 500,3 500 lx).The main results were as follows:1.Light intensity influenced the OCR and AER significantly(P<0.05).The oxygen consumption rate of the crab under 200-1 500 lx was significantly higher than those of the crab under 0 lx and 3 500 lx(P<0.05).The ammonia excretion rate of the crab under 1 500 lx was significantly lower than others(P<0.05).2.Light intensity influenced total protein,but did not influence other energetic contents and O:N ratio significantly(P>0.05).Our results suggest that protein is used preferentially as energy source.3.Marked differences were found in LD(P<0.05).The LD in muscle of the crab under 1 500 lx was lowest.4.Different light intensity influenced different respiratory enzymes differently.Under 1 500 lx,the activity of HK and PK were at a high level.Glycolysis went faster and produced more energy.The activity of SDH was at a high level,while LDH was low.Aerobic metabolism was at a higher level,providing more energy for a continuous motion of crab The results revealed that light intensity affected respiratory metabolism,and the crab at 1 500 lx had higher metabolism while under less stress.So about 1 500 lx may be the better illumination condition of the crab culture industry.

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王馨,王芳,路允良,董双林.光照强度对三疣梭子蟹呼吸代谢的影响[J].水产学报,2014,38(2):237~243

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  • 收稿日期:2013-10-08
  • 最后修改日期:2013-11-29
  • 录用日期:2014-02-20
  • 在线发布日期: 2014-02-20
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