Seven populations of Litopenaeus vannamei were collected to evaluate the general combining ability and the specific combining ability in harvest body weight and survival with the mixed linear model and generalized linear models,combined with constrained maximum likelihood method.Incomplete diallel crosses and nest design were adopted and 130 full-sib families were obtained by artificial insemination.The results showed that the populations of UA5,UA4 and SIN were higher than others in general combining ability of harvest body weight,the magnitude were 0.40,0.32 and 0.19,respectively.The general combining ability of harvest body weight and survival in the hybrid groups of UA5×SIN,UA1×UA2,UA4×UA5,UA4×SIN and UA1×SIN,which were priority combinations in producing superior offspring,had positive advantages.The analysis of variance of combining ability showed that in different environments,the proportion of GCA and SCA variance component in body weight traits had little difference.Correlation analysis showed that there is positive correlation between the GCA of harvest weight,but negative correlation in survival.For the study of general combining ability and specific combining ability in different groups,the experiment provided the basic data for further genetic breeding of L.vannamei.