FcβGBP-HDL was a pattern recognition receptor(PRR)which was up regulated post WSSV(white spot syndrome virus)challenge.To investigate the mechanism of transcription regulation,primers were designed based on the FcβGBP-HDL cDNA obtained,the gene and 5′-flanking sequences were isolated from the Chinese Shrimp Fenneropenaeus chinensis by genome walking,and the activity of promoter was analyzed by voter construction.The results showed that this gene was composed of only one exon spanning 6 713 bp.The 5′-flanking sequence(1 507 bp)of FcβGBP-HDL gene contained several typical transcription regulation elements,such as one promoter core sequence,two SRF,two TBP,one CTF and one CRE,and several transcription regulation elements of immune-gene in other arthropods,such as two GATA 1,three AP 1 and seven Sp1.The upstream sequence of FcβGBP HDL gene had highest level similarity with FcCTL(similarity 41.4%).The transcription levels of luciferase reporter gene were up regulated in all construction voters,highest in pGL3-FcβGBP2 with the deletion of two AP-1,and lowest in pGL3-FcβGBP4 with the deletion of promoter core sequence.The results indicated that promoter of FcβGBP-HDL might be regulated in immune reaction.