This study focused on evaluating the effects of different dietary lipid sources on growth, body composition, serum biochemical indices, and activities of lipometabolism enzymes of Carassius auratus gibelio. The growth experiment was conducted to test four dietary lipids (soybean oil, rapeseed oil, linseed oil, and fish oil) on C. auratus gibelio. The fish with initial body weight (14.33±0.02) g were randomly divided into 4 groups with 3 replicates per group, fed with 5.4% of each lipid included in basic diet in a recirculation system for 63 days. The results showed that the weight gain rate and the specific growth rate of fish fed both soybean oil and rapeseed oil were significantly (P<0.05) higher than that of fish fed fish oil, while the condition factor of fish that fed soybean oil was significantly (P<0.05) lower than that of fish fed fish oil and linseed oil, and not different from that of other groups. However, the muscle moisture, the crude lipid, the crude protein, and the ash content in different tissues of C. auratus gibelio did not showed significant difference among the groups (P>0.05). Fatty acid composition of muscle in fish generally reflected the composition of the diet. There was close correlation between the fatty acid composition of fish muscle and those of experimental diets. The activities of glutamic-pyruvic transaminase (GPT), glutamic-oxalacetic transaminease (GOT), alkaline phosphatase (ALP) in the plasma of fish oil group were significantly higher than the other groups (P<0.05), while no significant difference was found among all treatments in insulin, cortisol, total protein (TP), albumin (ALB), globulin (GLB), A/G, glucose (GLU), cholesterol (CHOL), and triglyceride (TG) in the plasma. Furthermore, enzyme activities related to lipid metabolism (carnitine palmityl transferase-I, carnitine palmityl transferase-II, acetyl-coa carboxylase, lipoprotein lipase and so on) of crucian carp were not significantly different among all treatments. These results indicate that the diet supplemented with rapeseed oil or linseed oil in feeding C. auratus gibelio can also achieve good growth and provide theoretical basis for the replacement of fish oil and soybean oil, which can economize the production cost in the future.