A small amount of yellow shell color of small abalone were found in the stock of “Dongyou No.1”.In order to understand the relationship between shell color and growth traits,four different color lines of Haliotis diversicolor including BB(normal♀× normal♂),YY(yellow♀× yellow♂),YB(yellow♀× normal♂)and BY(normal♀× yellow♂)were established by inbreeding and crossbreeding.The biological parameters of egg dismeter,fertilization rate,hatching rate,metamorphosis rate,survival rate and growth traits among different shell color lines were studied.Genetic diversity and genetic structure for the four lines were compared using 16 microsatellite markers.The results showed that yolk diameter of yellow shell color line was significantly larger than that of normal shell color line(P<0.05).Egg diameter,fertilization rate,hatching rate,metamorphosis rate and survival rate showed no significant differences(P>0.05)among BB,YY,YB and BY lines.At 90 days after fertilization,the growth of shell length and width among the four lines showed no significant differences(P>0.05).The results also showed that BB line had the lowest genetic diversity.In addition,Nei’s genetic identity(0.632 9-0.957 1)and genetic distance(0.043 9-0.457 3)indicated that genetic identity between BB and YY(0.632 9)was the lowest and genetic distance between them was the farthest(0.457 3).The results indicated genetic differentiation exists between normal and yellow color individuals.The present study provides basic data and theoretical basis for selective breeding of the small abalone using shell color as a candidate marker.