High content of cadmium in laver has seriously affected the edible safety,export and industry development of seaweed in our country.However,only total cadmium content was detected by present method used worldwide and the total cadmium was used to evaluate the food safety of the laver which may overestimate the toxicity of the cadmium in laver.Because there are different cadmium forms in laver which have different biological toxicities,the speciation analysis of cadmium in the laver is very urgent.However,studies on the cadmium speciation analysis in seaweed are very scarce at present.In present experiment,we first analyzed the different cadmium forms in laver(Porphyra haitanensis and Porphyra yezoensis) which have high Cd accumulation capability by using size exclusion chromatography-high performance liquid chromatography-inductively coupled plasma mass spectrometry(SEC-HPLC-ICP-MS).The result showed that three Cd species including Phytochelatins(PC)₃-Cd,glutathione(GSH)-Cd and one small molecule Cd species were detected in the deionized water extraction of the laver. In vitro wholebionic digestion model was used to determine the main cadmium forms in laver under the action of saliva,the acidity of stomach and intestine with inorganic and organic components and digestion enzymes.Two unknown Cd species with small molecule were detected in whole-bionic digestion of gastric extracts,and the retention time of main species was 24.2min.Two Cd species was detected in whole-bionic digestion of intestine extracts,and the main species was(PC)₃-Cd.However,further study is needed to study the metabolic characteristic of(PC)₃-Cd in the biology.Present study further proved the main organic cadmium species in seaweed and this has provided important basis for food safety evaluation of the laver.