Two salinity concentrations(salinity 12,salinity 25)and a control group of fresh water were set up in the experiment.The samples were respectively taken at 0,3,6,12,24,48,72,96,and 144 h to study the effects of salinity changes on the hemolymph biochemical parameters of the female parent Chinese mitten crab (Eriocheir sinensis).The results showed that the values of total protein(TP) in salinity concentrations were significantly higher than that of the control group during the first 6 hours(P<0.05)while the values of TP in two salinity concentrations reached the same level and kept a little higher than that of the control group after 96 hours.The values of glucose(GLU)increased with the increasing of salinity concentration,and the values of GLU both increased first and then declined in the salinity concentrations.The values of GLU in salinity 25 reached the maximum level at 72 h and were significantly higher than that of the other two groups (P<0.05).The values of triglycerides(TG)in salinity 12 declined gradually,and were significantly lower than that of control group during 6-144 h(P<0.05),yet were significantly lower than that of salinity 25 after 72 h(P<0.05).The values of TG in salinity 25 declined first and then increased,and reached the same level as control group after 72 h.The values of alkaline phosphatase(ALP)both increased first and then declined in the two salinity concentrations.The values of lactate dehydrogenase(LDH) in the two salinity concentrations both reached the maximum values at 3 h and reached the minimum at 12 h.The result showed hemolymph TP,GLU,and TG all changed significantly during travelling from fresh water to saline water in parent Chinese mitten crab.It could be concluded that(1) the parent Chinese mitten crab enhanced the process of energy metabolism to deal with the sudden change of salinity and increased the use of lipids and carbohydrates as energy source.(2) the parent Chinese mitten crab first regulated protein metabolism in response to the changes of external osmotic pressure and finally adapted to the high-salinity water.