In this study,full-length cDNA sequence of heat shock protein 70(HSP70) was isolated from hepatopancreas of Exopalaemon carinicauda,which was named Ec-HSP70.The fulllength cDNA sequence of heat shock protein 70 is 2 250 bp,which contains a 83 bp 5′-UTR(untranslated region),208 bp 3′UTR(untranslated region),and 1 959 bp open reading frame(ORF) that encodes 652 amino acid residues.There are three HSP70 family signatures between 8-15,199-206 and 334-348 amino acids residues of Ec-HSP70,and these sequences are highly conserved in Fenneropenaeus chinensis,Litopenaeus vannamei,Penaeus monodon,Marsupenaeus japonicus,Metapenaeus ensis.The phylogenetic analysis based on amino acid sequence shows that Ec-HSP70 has the highest similarity(99.8%) with HSP70 of L.vannamei and F.chinensis, and has above 90% similarities with other crustacean species,indicating that this protein is one of heat shock protein HSP70 family members.The expression of the gene in hepatopancreas and muscle was analyzed by quantitative Real-time PCR,the result shows that the expression of HSP70 gene was higher in hepatopancreas and muscle after stimulation with the tempreture,pH and NH4Cl.The higher expression time of Ec-HSP70 cDNA in hepatopancreas was earlier than in muscle.HSP70 mRNA expressions were inhibited with longer stress time.Hepatopancreas were more sensitive than muscle to the tempreture,pH and NH4Cl stresses.