Polyodon spathula cartilage as the cartilage tissue mainly contains two kinds of functional components:protein and polysaccharide,which possess antitumor,anti-aging effects and so on.The extraction,structure as well as activities of polysaccharides from the cartilage were investigated extensively and deeply.However,the extract technology for protein-polysaccharide complex has less been reported compared with polysaccharides.Therefore,the present work uses Polyodon spathula cartilage as material,and focuses on the process of protein-polysaccharide complex extracted by microwave-assisted alkali.The effects of alkali concentration,microwave power,microwave time and the ratio of liquid to material on the extract yield of protein-polysaccharide complex were studied by single factor analysis.On the basis of single factor analysis,the orthogonal test was performed to optimize the extract technology of microwave-assisted alkali.The results indicated that the optimum conditions of protein-polysaccharide complex from Polyodon spathula cartilage were cartilage with the ratio of liquid to material(15∶1, V/W),was extracted by 0.9% alkali solution,and was synchronously treated with 360 W microwave power for 6 min to obtain extract yield of 15.72%.The extraction technology of the microwave-assisted alkali can reduce the consumption of alkali,shorten the extraction time,and enhance the extract yield of cartilage.This work is favorable to further purification of proteins and polysaccharide component from Polyodon spathula cartilage,as well as to development of new health food and medicinal function products by providing the technical basis.