In the present study,female marbled eel(Anguilla marmorata) were induced to develop ovarian maturation by repeated injection of carp pituitaries extract(CPE) and LHRH-A-2. Five in the fifteen randomly selected injected eels were matured,and were in the completely mature stage with the mean GSI of (20.9±5.04)%(the max GSI was 27.3%); five were in the fullgrown stage with the mean GSI of (7.2±2.86)%; five were in the vitellogenic oocytes stage with mean GSI of (2.32±1.5)%;while the controls were in theⅡ stage with the mean GSI of (1.18±0.39)%.The result showed that eels could be induced completely mature by the exogenous hormone management.mRNA levels of pituitary GtH subunits,serum steriod levels,and histology of hepar and alimentary canal of eels in different development stages were examined.Quantitative realtime PCR revealed that mRNA expression levels of GtHα,LHβ and FSHβ subunits increased with the oocytes development and then decreased after the maturation of ovary.The GtHα and LHβ levels decreased with the maturation of ovary,but were still highly expressed.While the FSHβ levels were hardly to be detected in the matured eels.Serum E₂ levels were high in the early stages and rapidly decreased with the development of the ovary; Serum T levels increased during the ovary development,especially at the mature stage.The PAS stain reaction of hepar showed that the glycogen decreased with the ovary development,and the lipid droplets increased significantly.This can be directly attributed to the fact that glycogen in hepatocytes was involved in metabolism of matter and energy in the development of ovary.The histology of stomach and intestine wall showed varying degrees of degeneration during the development of ovary.