不同饲料对银鲳幼鱼增重率、肝脏脂酶及抗氧化酶活性的影响
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中央级公益性科研院所基本科研业务费(东2008M14,东2009M05);沪农科攻字(2007)第4-1号


Effects of different diets on weight gain,hepatic lipase and antioxidant enzyme of juvenile silver pomfret(Pampus argenteus)
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    摘要:

    利用营养学与生物化学的方法,研究分析了不同饲料对银鲳幼鱼增重率、饲料系数、肝脏脂酶及抗氧化酶活性的影响。试验共设4组不同饲料,依次为饲料1(新鲜鱼肉糜)、饲料2(新鲜鱼肉糜+饲料)、饲料3(新鲜鱼肉糜+饲料+蛏子肉糜)和饲料4(新鲜鱼肉糜+饲料+蛏子肉糜+桡足类)。试验用银鲳幼鱼的平均体重为(4.80±0.11)g,每组饲料设3重复,试验周期为9周。研究结果显示,不同饲料可显著影响银鲳的增重率,各饲料组中以饲料1组的增重率最低,并显著低于其它各饲料组;饲料4组的增重率最高,并显著高于饲料2、3组的增重率(P<0.05)。饲料系数以饲料1组最高,且显著高于其它3组饲料组;饲料2、3、4组饲料系数间并无显著性差异(P>0.05)。饲料1组肝脂酶与总脂酶活性均显著低于其它3组饲料组(P<0.05),但饲料2、3、4组间肝脏脂蛋白脂酶、肝脂酶和总脂酶活性均未有显著性差异(P>0.05)。各组间肝脏超氧化物歧化酶(SOD)与谷胱甘肽过氧化物酶(GSH-PX)活性均未有显著性差异(P>0.05),但均以饲料1组最低、饲料4组最高;饲料1、2、3组间肝脏过氧化氢酶(CAT)活性未有显著性差异(P>0.05),但饲料4组CAT活性显著高于饲料1组的CAT活性(P<0.05)。分析结果表明,单纯利用鱼肉糜作为饲料不利于银鲳的生长;饲料中添加桡足类不但有助于促进银鲳的生长,也可提高其抗氧化能力。

    Abstract:

    A 9week feeding experiment was carried out on juvenile silver pomfret Pampus argenteus[initial weight,(4.80±0.11)g] to evaluate the effects of feed composition on weight gain,feed conversion ratio(FCR),hepatic lipase and antioxidant enzyme activities using nutriology and biochemistry.Four diets were formulated and named diet 1(fresh fish meat),diet 2(fresh fish meat mixed with formulated feed),diet 3(fresh fish meat mixed with formulated feed and solen meat)and diet 4(fresh fish meat mixed with formulated feed,solen meat and copepods).Each diet was assigned to triplicate groups.The results showed that the weight gain of silver pomfret could be affected significantly by the feed composition.The weight gain of diet 1 was the lowest among the four diets,and was significantly lower than those of other three diets.The weight gain of diet 4 was the highest,and was significantly higher than those of diet 2 and diet 3(P<0.05).The FCR of diet 1 was the highest among the four diets,and was significantly higher than those of other three diets.There was no significant difference in FCR among diet 2, diet 3 and diet 4(P>0.05).Hepatic lipase and total lipase activities of diet 1 were significantly lower than those of other diets(P<0.05),but no significant difference was found in hepatic lipase,lipoprotein lipase and total lipase activities among diet 2,diet 3 and diet 4(P>0.05).There was no significant difference in superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)activities among all diets(P>0.05).However,the lowest SOD and GSHPX activities were both in diet 1 and highest were in diet 4.The catalase(CAT)activity of diet 4 was significantly higher than those of diet 1,2 and 3(P<0.05),but no significant difference was found among diet 1,2 and 3(P>0.05).In conclusion,the only use of fish meat as a feed is not beneficial to the growth of silver pomfret.Copepods could be also used in the diet to improve the growth and antioxidant ability of silver pomfret.

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彭士明,尹飞,孙鹏,施兆鸿,王建钢.不同饲料对银鲳幼鱼增重率、肝脏脂酶及抗氧化酶活性的影响[J].水产学报,2010,34(6):769~774

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  • 收稿日期:2010-01-28
  • 最后修改日期:2010-03-31
  • 录用日期:2010-04-07
  • 在线发布日期: 2010-06-10
  • 出版日期: 2010-06-15