Ruditapes philippinarum has a widespread consumption in China. It is enjoyed for the uniqueness of its flavour and has a wide variety of applications in foods, such as soup and flavouring. Associated with the diversiform consumption of Ruditapes philippinarum is the general increase of allergic disorder. To provide guarantee for the allergy sufferers, it is important to establish a more convenient method for the detection of food allergen. A visible antibody microarray for detecting Ruditapes philippinarum allergen was developed in this study, which was based on the 3, 3′, 5, 5′Tetramethylbenzidine (TMB)H₂O₂ reaction catalyzed by horseradish peroxidase (HRP). Several physicochemical parameters such as the incubation time and the dilution ratio of antibodies were optimized. A modified sandwich reaction was performed with immobilization of rabbitanti-Ruditapes philippinarum antibody onto the threedimensional slide, followed by adding antigen，mouseanti-Ruditapes philippinarum，HRPgoatanti-mouse IgG. After incubating, TMB was employed to give signals, the slides were observed by naked eyes and recorded with flatbed scanner, analysed with GenePix Pro 6.0. The results showed that allergen from Ruditapes philippinarum can be detected sensitively with a sensitivity of 10 ng/mL. The method has good reproducibility with average intraassay CV of 5.9% and average interassay CV of 10.3%. The recoveries of three different spiked concentrations in sausage and surimi stick ranged from 73.54% to 95.44%. During storage at 4 ℃, the activity of the chips kept stable for 4 months. Moreover, some crossreactivity with Penaeus vannamei was found. With visible and stable results, this method has shown great potential in the massive parallel analysis of various food allergens simultaneously.