Lipovitellin(Lv) is one of the major proteolytic products of vitellogenin(Vtg) after it is taken up by growing ovary in the manner of receptormediated endocytosis，which provides nutrient storage for the developing embryos and larvae．In this paper，we purified the Lv from ovarian extract of the Pelteobagrus vachelli in stage Ⅳ by two steps of chromatography elution method．The first step of purification was to use Sephacryl-S-300 column，and 3 elution peaks were showed．We are suspicious that the 3th elution peak contained Lv through analysing the Native-PAGE chart and elution curve of stage Ⅳovarian extract．The second step of purification was to use DEAEcellulose column in ionexchange chromatography elution，and there were 2 elution peaks．We found that the 2th elution peaks(in 0.2 mol/L NaCl gradient) contained single high concentration of protein by Native-PAGE analysing of the sample．The Lv characterized as a phosphorlipoglycoprotein by NativePAGE and stained of gels for carbohydrates with periodic acidSchiff’s reagent，for phosphorus with Rhodamine B，and for lipids with Sudan black B．The molecular weight of Lv is about 230 ku detected by Native-PAGE．The Lv broke into 2 same subunits in Sodium dodecyl sulphatePAGE，each with a molecular weight of 106 ku．In 190-1 000 nm continuous spectrum scanning，the purified Lv of P. vachelli obviously showed absorb peak in 273 nm，and it demonstrated that the Lv contained carotenoid．NativePAGE analysis of the purified Lv of P. vachelli show that there is no disulfide bond．It is relatively stabilized by heated treatment．We prepared the rabbit polyclonal antiserum against P. vachelli Lv by using purified Lv．The titre for Lv polyclonal antisera was 1∶32 in double immunodiffusion assay detection．It was showed that the purified Vtg which was induced by E₂ in male P. vachelli serum could react with the rabbit antisera against P. vachelli Lv and the purified Lv could react with the rabbit antisera against P. vachelli Vtg and a single immunoprecipitin line was formed．The plasma from the control male P. vachelli have no reaction．The normal existence of the femalespecific reactivity for P. vachelli Lv can be confirmed．The test also demonstrated that both purified Vtg and stage Ⅳovarian extract from the P. vachelli can crossreact with the rabbit Lv polyclonal antiserum by the analyses of Native-PAGE and Westernblotting．We are sure that the rabbit Lv polyclonal antiserum has preferable specificity with Vtg and Lv．