The virus named AVNV (acute viral necrotic virus) can cause the mass mortality in cultured scallops Chlamys farreri. So the research of AVNV vertical transmission is necessary. In the experiment, the study put the parental scallops into the separate breeding waters, then spawning, hatching, and cultivating larvae. According to the artificial spring and autumn seed of scallop indoors, from the forty separate breeding water bodies, 23 parental scallops, 23 eggs, 12 spermoviums, D larvae and shell young, 2 eyebot larvae were obtained. For the qualitative and localized detection, the methods of Indirect ELISA, IFA and Electron microscope were used to detect parental scallops, eggs and the larvae with different development phases. The result shows that the virus can be detected in the gonad of the parental scallops, but it can not be detected in the eggs, spermoviums, D larvae, shell young, and eyebot larvae. This indicates that AVNV can infect the gonad of scallop (Chlamys farreri), but there does not exist the route of vertical transmission sensu stricto. For further study, the research of horizonatal transmission is essential.