To provide data for immunoglobulin (Ig) phyletic evolution, isotypes classification and vaccine used in Hucho taimen (Pallas) cultivation, cDNA sequence, gene diversity and physicochemical property on Ig VL (variable region of light chain) of Hucho taimen were studied. The cDNA clones of the immunoglobulin light chain variable regions were first time obtained by RTPCR with a pair of specific primers after total RNA was extracted from the spleen of Hucho taimen (Pallas). 51 positive clones were isolated and doublestrand sequenced over the variable regions. 46 complete sequences were obtained without duplicating to determine variety of Ig VL genes families. After comparing with other vertebrate Ig sequences in BLUST, the results show that the 46 sequences obtained are similar to the sequences of rainbow trout Oncorhynchus mykiss Walbaum, no. X68517.1 and no. X68519.1, reported in GenBank, with the similarity rate from the lowest 86.1% (that of P4 and P43) to the highest 99.8% (that of P21 and P38). Jotun Hein method from MegAling of DNAstar soft package was used to analyze amino acid homology of Ig VL and the VL genes were classified into two families. After computing method of variability parameters, it was found that CDRs (complementary determining regions) are the main variable regions, and hyper variability was showed in CDR3. By analysis of CDR3 sequences, a conclusion was drawn that character of rearrangement style of Ig VL of Hucho taimen is that Jκ genes are located at 3′side, and Vκ genes are followed. The rearranging way makes nucleotides link styles variable, and thus causes more diversity of the Ig. The hydropathic analysis showed that hydrophilic amino acids of VL are mainly distributed at forepart of LP, terminal of FR1, the whole FR2, amphisides of FR3 and foreparts of CDR1, CDR2 and CDR3.