Starry flounder (Platichthys stellatus) is the large carnivorous fish and demands diet with high level protein. This experiment was carried out to study the effect of replacing fishmeal with new protein sources in diets of juvenile starry flounder ［initial weight, (75.6±0.18) g］. Five isonitrogenous and isoenergetic diets (D0, D17, D35, D52 and D69) were formulated by regulating amino acid level with new protein resources (soy protein concentrate, dephenolized cottonseed protein, spraydried blood meal, etc). D0 with 58% defatted fishmeal as the control group, fishmeal was replaced by new protein sources at four levels of 17%, 35%, 52%, 69%. Five groups in triplicate were divided randomly for the 60day experimental period. At the end of the experiment, growth performance, body composition and partial immunological parameters were studied. The results show that weight growth rate(WGR), protein efficiency ratio (PER) and specific growth rate (SGR) of D0 are 141.84%，229.88%and 1.47%/d，respectively, no significant differences occurred among D17, D35 and D52 (P>0.05), but D69 is significantly low at 107.77%,186.37% and 1.22%/d (P<0.05), respectively；Feed conversion ratio (FCR) of D0 is 0.80 and not significant to D17, D35 and D52(P>0.05), but D69 is 0.98 and higher than the other four groups obviously(P<0.05)；Digestive tube index(DTI) tends to enhance with increasing level of new protein sources，D0 is 2.01% , significantly lower than D69(P<0.05); Condition factors (CF) has no significant difference (P>0.05) in five groups. There are no significant differences (P>0.05) in the contents of protein and fat in whole fish and muscle，while the mineral content in whole fish increases with the replacement of fishmeal；erythrocyte, leukocyte，hemoglobin and serum protein have no significant difference(P>0.05) among groups，but increase of new protein sources has some negative impact on hematocrit(HCT) and lysozyme activity. These results show clearly that dietary fishmeal levels can be considerably reduced without any adverse effect on growth performances for juvenile starry flounder. Digestive enzyme activities, amino acid and the utilization of minerals of juvenile starry flounder are the target of research at present.