青石爬鮡血浆生化指标、血细胞分类与发生
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广西壮族自治区科技厅项目(2008-0256)


Plasmic biochemistry indicators, classification of blood cells and haecytogenesis of wild Euchiloglanis davidi
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    对40尾野生青石爬鮡进行了血液生化指标、血细胞分类与发生研究。测得血浆的总蛋白为(48.35±3.60) g/L,白蛋白为(8.73±0.54) g/L,球蛋白为(39.63±3.10) g/L,血浆白蛋白与球蛋白比为(0.22±0.01),甘油三酯为(5.05±2.14) mmol/L,总胆固醇为(16.66±3.64) mmol/L,极低密度脂蛋白为(1.01±0.43) mmol/L,葡萄糖为(5.27±2.43) mmol/L。血液生化指标检测结果表明,青石爬鮡血浆中总蛋白与球蛋白、甘油三酯与胆固醇较高,葡萄糖较低,具有活动量较大和冷水性鱼类的特点,主要利用脂肪和蛋白质作为能量。细胞学显示出其外周血液包含红细胞,核影,淋巴细胞,血栓细胞,嗜中性粒细胞和单核细胞。血涂片计数为红细胞96.58%,其中成熟红细胞占65.92%,幼稚红细胞占30.66%。白细胞为3.42%,其中嗜中性粒细胞0.14%、单核细胞0.07%、淋巴细胞2.37%、血栓细胞0.85%。白细胞中淋巴细胞和血栓细胞数量较多。原始血细胞主要在中肾和头肾中,在脾脏中也有部分原始白细胞,而肝脏和外周血液中未见原始血细胞。中肾和头肾是青石爬鮡的主要造血器官,其中红细胞主要由头肾产生,白细胞主要由中肾产生。脾脏亦产生少量白细胞。

    Abstract:

    40 wild Euchiloglanis davidi were studied on the plasmic biochemistry indicators, classification of blood cells and haecytogenesis. In the plasmic biochemistry indicators, there were (48.35±3.60) g/L total protein, (8.73±0.54) g/L albumin, (39.63±3.10) g/L globubin, (5.05±2.14) mmol/L triglyceride, (16.66±3.64) mmol/L cholesterol, (1.01±0.43) mmol/L very low density lipoprotein (VLDL), (5.27±2.43) mmol/L glucose in plasma. These indexes indicated that albumin, globubin, triglyceride and cholesterol were positively high, but glucose negatively low in plasma of freshwater fish. These characteristics showed that E. davidi has large activity amount and is coldwater fish, which utilizes lipid and protein as main energy resource. In corpuscle of the fish, there were erythrocytes, ghost cells, lymphocytes, thrombocytes, neutrophilic granulocytes and monocytes to be found. By counts with blood smears, there were 96.58% erythrocytes (therein, 65.92% erythrocyte and 30.66% erythroblast)and 3.42%leucocytes (therein, 2.37% lymphocytes, 0.85% thrombocytes, 0.14%, neutrophils and 0.07% monocytes). Most original corpuscles were found in pronephros and mesenephros and some original leucocytes were also found in spleen, but no original corpuscles were found in liver and blood. In E. david, pronephros and mesenephros are the principal hematopoietic organs. Erythrocytes mainly come from pronephros and leucocytes mainly come from mesenephros. Some leucocytes are also produced in spleen.

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冯健,杨丹,覃志彪,杜卫平,蒋步国,潘艳云.青石爬鮡血浆生化指标、血细胞分类与发生[J].水产学报,2009,33(4):581~589

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  • 收稿日期:2008-08-02
  • 最后修改日期:2009-02-02
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  • 在线发布日期: 2009-07-04
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