We analyzed the nutritive compositions of 3 preys (Acanthogobius ommaturus, Exopalaemon annandalei, Corbicula fluminea), artificial feed, wild and farmed juvenile Acipenser sinensis. By calculating the ratio of essential amino acids in the 3 preys and artificial feed to that of wild and farmed juvenile A. sinensis (a/A) and essential amino acid index (EAAI), we evaluated the protein nutritive value of the 3 preys and artificial feed. By analyzing the fatty acid contents of 3 preys, artificial feed, wild and farmed juvenile A. sinensis, we evaluated the fatty acid nutritive value of the 3 preys and artificial feed. The results showed that the content of crude protein was 80.57%, 67.84%, 51.88%, 54.20%, 91.83%, and 83.40% respectively. Using the muscle of wild juvenile A. sinensis as the reference protein, EAAI of 3 preys and artificial feed was 0.938, 0.913, 0.918, and 0.956 respectively. And using the muscle of farmed juvenile A. sinensis as the reference protein, EAAI were 0.940, 0.913, 0.915, and 0.953 respectively. Protein analysis of 3 preys and artificial feed showed that they are all highquality protein material for the wild and farmed juvenile A. sinensis. By analyzing the fatty acid contents of 3 preys and artificial feed, we found that the contents of n-3 PUFA were rich in A. ommaturus, E. annandalei and C. fluminea, but were poor in artificial feed. n-3 PUFA in artificial feed could not meet the needs for the migration of juvenile A. sinensis. As the nutritive compositions of 3 preys and artificial feed are different, the selection and development of artificial feed for juvenile A. sinensis according to the natural nutritive compositions characteristics of their preys are quite necessary and we should supplement the needed nutritive components to the artificial feed in order to meet the different needs of their survival, growth and migration.