An experiment was conducted to study the effects of dietary vitamin E on growth（weight gain rate，feed efficiency ratio，specific growth rate and survival rate），vitamin E concentration in tissue and immune responsed（lysozyme activity and total complement activity）of Japanese seabass（Lateolabrax japonicus）. Five iso-nitrogenous and iso-energetic diets were formulated to contain graded level of vitamin E (0.6，24.7，49.0，98.9，and 198.2 mg•kg-1 diet，respectively ) and fed to15 floating netcages（1.5×1.0×1.0 m）with 20 fish (initial weight: 10.5g) each twice daily（09:30，16:30）for 8 weeks. Results indicated that after 8 weeks of the feeding trial，weight gain rate，feed efficiency ratio，specific growth rate and survival rate of the fish fed with the control diet were significantly lower than those with vitamin E supplemented diets. The weight gain rate and specific growth rate increased significantly with increasing dietary vitamin E from 0.6 to 49.0 mg•kg-1 and the optimal vitamin E content for maximum growth is about 60.5 mg/kg；Moisture and fat concentration in whole body of Japanese seabass were not affected by the supplemental levels of dietary vitamin E，but the protein concentration increased significantly with increasing dietary vitamin E from 0.6 to 49.0 mg•kg-1，Meanwhile，the vitamin E concentration in liver and musle increased significantly with increasing dietary vitamin E from 0.6 to 49.0 mg•kg-1, but no significant differences were observed among fish fed the diets with equal to or higher than 49.0 mg•kg-1 of vitamin E. The serum lysozyme activity and total complement activity of the fish fed with the control diet were significantly lower than those with vitamin E supplemented diets，the serum lysozyme activity and total complement activity of Japanese seabass increased significantly with increasing dietary vitamin E supplement，and the Japanese seabass had the maximal level of lysozyme activity and total complement activity when fed 98.8 mg•kg-1. Based on the information above, the optimal dietary vitamin E level for optimum growth performance and immune responses in Japanese seabass is about from 60.5 to 98.8 mg•kg-1.