This paper investigated the lipid contents and fatty acid compositions of ovary, liver and muscle of the wildcaught broodstocks of Pampus cinereus in IV stage of ovary development with body weight from 408 g to 614 g from the East China Sea. The results indicated that there were significant differences of lipid contents and fatty acid compositions among ovary, liver or muscle tissues. The average lipid contents of ovary, liver and muscle of the broodstocks were 45.10%, 30.61% and 19.56% respectively. The lipid content in ovary was relatively stable while in liver and muscle was significantly variable among different broodstocks. There was a positive interrelation of lipid content between liver and muscle. The main fatty acids in ovary of the broodstock were as follows:C16:0>C22:6n3>C18:1n9>C16:4n3>C18:0. While C16:0>C18:1n9>C22:6n3>C18:0>C14:0 in muscle and C16:0>C18:1n9>C18:0>C22:6n3>C14:0 in liver respectively. The contents of DHA (22:6n3) and EPA (C22:5n3) of ovary, liver and muscle were 18.887% and 3.268%, 5.856% and 0.707% and 9.672% and 2.407% respectively. The compositions of C16:4n3，C22:6n3，PUFA，HUFA in ovary were significant higher than those in muscle and liver. The ratios of DHA/EPA/ARA in ovary, muscle and liver were 5.90/1/0.44, 4.03/1/0.50 and 8.51/1/0.85 respectively. The ratios of n-6PUFA/n-3PUFA in ovary, muscle and liver were 5.89, 3.51 and 4.67 respectively. Therefore these data will be useful for the artifical cultivation of the broodstocks of Pampus cinereus.