Diseaseresistant thoroughbred breeding is considere d to be a feasible means to control white spot syndrome of cultured shrimps. For a better understanding of the immunogenicity and WSSV resistance ability of ant iWSSV Penaeus japonicus F3, so that some constructive inform ation can be obtained to improve the breeding of diseaseresistant shrimp, the antiWSSV P. japonicus F3 and the healthy normal shrimps were injected with white spot syndrome virus(WSSV) suspension with a density of 1×1 05 virus per mL. The mortality of the injected shrimp was recorded and the hem olymphs of shrimps from both groups were collected at 0, 4, 24, 48, 96 h posti njection for the evaluation of the immunoparameters such as PO, ACP, AKP, SOD an d protein concentration. The results showed that: the mortalities of antiWSSV F3 and the control shrimp were 15% and 85% at 14 d postinjection, respective ly, and the protective rate of antiWSSV F3 was increased by 82.4%; before in jection, the values of all of the immunoparameters in the hemolymphs of the anti WSSV P. japonicus F3 were higher than those of the control shr imps, and the values of PO and AKP activities as well as the protein concentrati on exhibited extremely significant difference (P＜0.01) from tho se of control group; five immunity indices exhibited the similar trends with the values of the antiWSSV F3 always higher than those of the control shrimps e xcept ACP. The results indicate that antiWSSV P. japonicus F3 can effectively protect themselves from WSSV by the stronger immune function. The results also indicate the promising future of further diseaseresistant tho roughbred breeding of P. japonicus.