Chinese shrimp (Fenneropenaeus chinensis) is one of the most important farmed species in China. During 1986-1992, China was a world leader in both the artificial propagation of shrimp larvae and harvest of cultured shrimp. However, the epizootic breakout in 1993 caused a serious decrease in the total harvest of cultured shrimp. One of the measures was to genetically improve the farming species. Many molecular genetics of F. chinensis were carried out to accelerate the breeding programs such as the analysis of mitochondrial genome and population genetic structures, screening of genetic markers linked to growth traits, etc. While the construction of the linkage map has been relatively slow. In the study, the primary genetic linkage maps of F. chinensis were constructed using “two way pseudo testcross” strategy with RAPD，SSR and AFLP markers. Parents and F1 progeny were used as segregating population. 61 RAPD primers, 20 pairs of microsatellite primers screened from 460 RAPD primers and 44 pairs of SSR primers combined 88 pairs AFLP primers were used to analyze the parents and 82 progeny of the mapping family. 783（237 RAPD, 45 microsatellites and 501 AFLPs）were segregating markers in which 761 markers were used to analysis. The female linkage map included 40 linkage groups, 15 triplets and 20 doublets, spanning 2835.5 cM with the average marker density of 12.5 cM, and the observed coverage was 73.5%. The male linkage map included 41 linkage groups, 6 triplets and 12 doublets, spanning 2776.7 cM, and the observed coverage was 73.3%. In addition, 5 microsatellite markers were mapped in both female and male linkage map that can be used for the construction of sexual linkage map of F. chinensis. Genetic linkage maps play a prominent role in many areas of genetics, including QTL analysis, mapbased cloning of genes, marker assisted selection (MAS) and comparative genomics. The main purpose of the study was to provide a theory basis for the construction of high consistency linkage map and promote the progress of genetic breeding programs of F. chinensis.