A cDNA library of Vibrio alginolyticus constructed by restriction display-PCR
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摘要: 摘 要: 应用限制性显示PCR(RD-PCR)技术构建溶藻弧菌poly（A）化mRNA的cDNA文库，筛选，收集cDNA片段，并进行测序，根据测序结果进行生物信息学分析和结果验证。实验结果表明，我们成功地构建了溶藻弧菌poly（A）化mRNA的cDNA文库，对其中53个基因片段进行分析，获得细菌Ⅲ型分泌系统易位蛋白、趋药性传感器、类丝氨酸蛋白酶等基因片断。说明了我们所构建的溶藻弧菌poly（A）化mRNA的cDNA文库质量高，本文并探讨了限制性显示PCR技术在细菌poly（A）化mRNA的cDNA文库构建中的应用价值。 关键词：cDNA文库；限制性显示PCR；溶藻弧菌
Abstract: Abstract: A cDNA library of Vibrio alginolyticus mRNA with poly(A) tracts was constructed and identified by restriction display- polymerase chain reaction (RD-PCR). After being selected and screened, bioinformatics of 53 clones were analyzed. The results showed that some genes such as translocation protein in type III secretion, type III secretory pathway component EscU, putative chemotaxis transducer and secreted trypsin-like serine protease were identified, which indicated a high quality cDNA. The applied value of RD-PCR in construction of cDNA library of Vibrio alginolyticus mRNA with poly(A) tracts was also analyzed in this research. Key words:cDNA library; restriction display-PCR(RD-PCR); Vibrio alginolyticus
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王 蓓, 吴灶和,简纪常,鲁义善.应用RD-PCR技术构建溶藻弧菌的cDNA文库[J].水产学报,2007,31(6):731~736
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收稿日期:2007-07-03
最后修改日期:2007-07-03
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在线发布日期: 2007-10-28
出版日期: 2007-11-16
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