A 8-week feeding experiment was conducted to evaluate effects of dietary supplementation of housefly larva meal and β-glucan on growth and immunity of Litopenaeus vannamei. Five isonitrogenous and isoenergetic diets (D1-D5) were formulated. D1, a practical diet, was served as a control. D2 and D3 were formulated respectively replacing 5% and 3% fish meal with housefly larva meal. D4 and D5 were formulated respectively replacing 0.5% and 1% wheat flour with βglucan preparation containing 25% β-1,3-glucan. Each diet was randomly assigned to 3 replicate cages (33 cm×80 cm×100 cm), which were set up in a 12 m3 indoor tank with a recirculation system. 60 juvenile shrimp (mean body length 1.5 cm) were stocked in each cage. The shrimp were fed to apparent satiation four times daily. At the end of the feeding experiment, body weights, survivals and feed conversion ratios were calculated, and blood samples from each group were collected to determine serum nonspecific immunological parameters and biochemical parameters. At the same time, a challenge test was conducted among some of the shrimp from each treatment by abdominal injection of Vibrio alginolyticus. The results of the feeding experiment showed that there were no significant differences in the body weight, survival rate and feed conversion ratio (FCR) between the treatments (P＞0.05). The examination of some nonspecific immunological activities in serum revealed that there were significant differences (P＜0.05) in phenoloxidas (PO) activity while there was no rule to be found, and there were no significant differences (P＞0.05) in total superoxide dismutase (SOD) activity, and that D1 led to a significantly higher lysozyme activity than the other treatments (P＜0.05). The serum biochemical indexes indicated that the values of glutamatepyruvate transaminase (GPT) and lactate dehydrogenase with D1 treatment were significantly higher than those of the others (P＜0.05). The challenge test showed that the first twoday accumulative mortality with D1 treatment was much significantly higher than those with the others (P＜0.01), and then the mortalities tended to be stable after the fourth day. The final accumulative challenge mortality (53.3%) of D1 treatment was significantly higher than that (33.3%) of D2 treatment and that (37.8%) of D5 treatment (P＜0.05). This study suggested that dietary supplements of housefly larva meal and βglucan enhanced disease resistance of Litopenaeus vannamei to vibrio to some extent, but did not exert positive effects on the growth and feed conversion.