Inbred and hybrid lines were established based on two different broodstocks of Litopanaeus vannamei, named as stock A and B. Stock A was SPF prawns introduced from America Oceanic Institute(Hawaii), stock B without specific virus was selected through many generations from Hainan local shrimp whose parents were coming from Taiwan. Inbred line consisted of two groups AA（A♀×A♂）, BB（B♀×B♂）. Hybrid line consisted of two groups AB（A♀×B♂）, BA(B♀×A♂). Comparing larvae growth and survival of F₁ from different line under the same hatchery and nursery conditions, we find that the growth rate of inbred group is AA>BB, the survival of inbred group is BB>AA. The result shows that there are significant differences: group AA grow fast, group BB have higher survival rate. In hybrid groups, the growth is AB>BA and the survival rate is BA>AB. For inbred and hybrid groups, the growth rate is AA>AB>BA>BB, the survival rate is BB>BA>AB>AA. Groups AB and BA of hybrid line show the double supeior phe notype trait of growth and survival rate, which implies hetetosis is obvious. And the hybrid progeny inherit the parent phenotype trait, but in terms of growth and survival, the heritability of parent leading.