Biofilter technology plays a key role in the production process of recirculating system. The establishment of biofilter＇s full nitrifying function is very important to avoid the aquacultural hazards for producers. There were many reports about the cultivation of the biofilter in the recirculating system. However, almost all of them were carried out in the lab scale and had little meaning to the production scale. This paper compared three methods of cultivating biofilter in the recirculating aquacultural system. The results showed that putting appropriate load fish directly and feed lowly was the best method to establish the nitrification function of the biofilter in the production of the recirculating aquaculture system.