Whitish muscle diseases of freshwater giant prawn, Macrobrachium rosenbergii, is a new epidemic disease in the larvae and post-larvae of M. Rosenbergii in recent years. The clinical sign of the diseam is a whitish or opacity appearance of the muscles in abdomen. Mortalities may reach more than 60%, sometimes even reach 100%. The pathogen of the disease is confirmed as Macrobrachium rosenbergii Nodavirus（MrNV）, an icosahedral and non-enveloped virus sized from 25 - 26 nm with two linear ssRNA sized 3.0 kb and 1.3 kb respectively. MrNV was the first nodavirus founded in crustacean. To develop a rapid method for MrNV detection, monoclonal antibody against MrNV was prepared and used for virus detection. Briefly, mouse myeloma .cells（SP2/0） were fused with spleen cells from BALB/c immunized with purified MrNV from diseased postlarvae（PL）. Positive colonies were screened by indirect ELISA with the plates coated with purified MrNV and cloned 2 - 3 times with limited dilution method. Twelve positive hybridoma cell lines secreting monoclonal antibodies （Mabs） against MrNV were at last obtained and used for ascites preparation. The titres of positive ascites Mabs were ranged from 1 ： 105 to 1 ： 106 by indirect ELISA. These twelve Mabs were isotyped by Southern Biotechnology clonotyping system, and the results showed that six Mabs were IgG1, four IgG2a and two IgG2b, respectively. No cross reactions, were found when the twelve Mabs were used for indirect ELISA with white spot syndrome virus （WSSV） and Taura syndrome virus（TSV）. A triple antibody sandwich enzyme-linked immunosorbent assay（TAS-ELISA） was developed with the plates pre-coated with rabbit polyclonal antibodies against MrNV for virus capturing, followed by MrNV and samples incubaticn, Mabs and goat-anti-mouse-IgG antibodies labeled with HRP. Mab 2B5, characterized as Mab against 43 kD coat protein of MrNV by Western blot, was selected for TAS-ELISA. The results showed that TAS-ELISA can be used successfully for minimum virus detection with the sensitivity of 0.98 ng MrNV, or with the viral titer of 1：40960 when typical diseased PL homogenization supematants were used for TAS-ELISA. TAS-ELISA could be considered as a useful technique for MrNV detection as well as for epidemiological studies for its rapid and inexpensive cost with high specificity and sensitivity.