In order to solve the problem of which food conversion ratio was higher and how nutritional disease such as fat liver occurred, when Monopterus albus fed formulated diet, this paper studied the effects of supplement of choline to the diets on the growth, food conversion ratio, lipid content in muscle and liver, hepatosmatic index and activities of 4 digestive enzymes of Monopterus albus. By adding choline of different content to the diets at 9 levels: 0.0%, 0.1%, 0.3%, 0.5%, 0.8%, 1.0%, 1.2%, 1.5% and 2.0%, cultivating M. albus for 90 d. Then the study measured and compared 9 levels of choline to the diets on the growth, food conversion ratio, lipid content in muscle and liver, hepatosmatic index and activities of 4 digestive enzymes of M. albus. The results show that when M. albus feed on diets with different content of choline, the growth rate, food conversion ratio, lipid content in muscle and liver, hepatosmatic index, activities of 4 digestive enzymes are different. Between 0.0% to 2.0% of supplement of choline to the diets, with the increase of choline content in the diets, its growth rate increases, the activities of protease, trypsin, amylase and lipase in foregut, hindgut and liver increase, and food conversion ratio,lipid content in muscle and liver and hepatoamatic index decrease. When the choline content in the diets increases from 0.0% to 0.8%-1.0%, these changes are significant. This suggests that the suitable supplement of choline in the diets is 0.8%-1.0%.