It is well known that the most pathogenic bacteria can produce various virulence factors and they play funct ion together and lead to the pathoenicity of bodis. The adhesion to cells or mucosa of pathogenic bacteria is the prime process which results in the infection of bodis. The adhesive factors of bacteria include pilus adhesion and non-pilus adhesion( such as f lagella, outer membrane proteins and hemagglusion etc. ) . The adhesion to tissuecells or mucosa of pathogenic bacteria mediating by pilus plays a key role in colonizing and propagat ing various virulence factors that result in the pathoenicity of bodis. In order to reveal the pathogensis mechanism of vibrio mimicus strain HX-4 in this study we used classic adhesive mode-l cell HEp-2 cells as cell model in vitro to test the adhesive ability of pathogentic vibrio mimicus HX-4 strain isolated f rom diseased Eriocheir sinensis to HEp-2 cells and the effect of receptors and antibody on the adhesion of the strain. At the same time, the invasive ability of the strain into HEp-2 cells was measured by lysiscounting assay. The results showed that vibrio mimicus HX-4 strain had invasive ability into HEp-2 cells, and it can adhere to HEp-2 cells in the way of assembly. That is to say vibrio mimicus HX-4 strain was positive bacteria of adhesion and the optimal adhesivetime was one hour. At the same time we also found that mannose and antibody could inhibit adherence effectively. These results demonstrated that there was D-mannose structure among the adhesive receptor structure and the adhesion of vibrio mimicus HX-4 strain may also include f lagella, outer membrane proteins besides pilus.