Penaeus vannamei juvenile was cultured making use of the system of treating circulation water with ozone and bio-filter indoor at estuary area. The circulation water was treated with ozone and bio-f ilter for 2h at 48 - 72h intervals, then aired for 2h. The water circulation among treating pond and shrimp ponds was temporarily stopped, filtering system continually ran during treating water. The quality criteria of the circulation water for the shrimp juvenile culture was controlled within suitable range for 52d. The average values of the criteria are as follows: NH3- Nt 0. 41mg#L- 1, NO- 2 - N 0. 057mg#L- 1, COD 10. 12 mg#L- 1, oxidat ion-reduction potential ( ORP) 379mv, turbidity 0. 6 NTU, bacterial total 10 200 cell#mL- 1, vibrio total 6cell#mL- 1, juvenile survival rate 78. 3% - 80. 2%. The mode of treating circulation water of indoor shrimp culture at estuary area is put forward according to the test result and the characteristic of estuary water.