鹧鸪菜再生苗育苗养成技术的研究
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THE TECHNIQUE OF ROPE-ADHERING SEEDLINGS OF CALOGLOSSA LEPRIEURII THALLUS REGENERATED FROM ITS TISSUE FRAGMNETS
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    摘要:

    本文研究鹧鸪菜微小组织块再生特性及其组织块再生苗附绳育苗养成技术。在组织块的切口处几乎每个细胞都具有分生能力。组织块的大小以 50~100个细胞左右为宜,这样大小的组织块再生植株数和再生率均表现出最高值。培养液的最适比重是1.015。培养液中添加5~50ppm壳多糖均能不同程度地促进组织块的再生能力。组织块的附绳固着率以100ppm壳多糖浸泡网绳培养结果最佳。组织块附绳后,在培养箱内平面静止培养15~20天,再生苗肉眼可见,再移养到较大水体水簇箱内冲气培养一个月左右,可成长为自然大小的成体。

    Abstract:

    The regeneration characteristics of the tissue fragments from Ca-loglossa leprieurii thallus were studied and the method of rope-adhering seedlings regenerated from tissue fragments was developed. Tha results are as follows:1 .The tissue fragments from v egetative leaches poasess great. capacity of rege-neration.2. The suitable site of the fragment is 50 to 100 cells.3. The specific gravity of the culture medium is 1 .010-1.025, the optimum va-lue is 1.015. 4.The proper amount of chitin added in the culture medium can enhance the regeneration and raise the adhension ratio of the pellets. Under ordinary tempera-ture and light for 15-20 days of culture, the regeneration buds became visible, after another 30 days of culture in a larger water body with aeration, the algae could grow to normal adult size. The method is easy to operate, and it will probably be-come a useful technique for seaweed production.

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刘凤贤.鹧鸪菜再生苗育苗养成技术的研究[J].水产学报,1990,14(3):219~226

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  • 收稿日期:1989-10-01
  • 最后修改日期:1990-03-01
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  • 在线发布日期: 2014-10-27
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