The research trend and application of rotifer mitochondrial genome research were proposed by searching, collecting, reading and summarizing relevant literature on rotifer mitochondrial genome research to understand the current situation and existing problems of rotifer mitochondrial genome research. Meanwhile, the research of analyzing and statistics of rotifer mitochondrial genome sequence were carried out. With the development of molecular biology technology, especially sequencing technology, research on mitochondrial genome sequencing in rotifers has gradually been carried out. Currently, there are 12 records of the complete mitochondrial sequence of rotifers reported in 6 species, mainly concentrated in bdelloid rotifers and rotifers of the genus Brachionus. The mitochondrial genome of bdelloid rotifers contains one ring chromosome, while the rotifers of the genus Brachionus contain two ring chromosomes. The mitochondrial genome of all rotifers contains 13 basic mitochondrial protein coding genes, 2 rRNA coding genes, and 22 tRNA coding genes (18-32). In addition, a few rotifers, such as B. calyciflorus, contain additional copies of the cytb gene. Due to the small size of rotifers and the difficulty of cloning and culturing, relatively few researchers, and the mitochondrial genome sequencing of rotifers needs to be further deepened. The sequences obtained from mitochondrial genome research can be used for research on phylogenetic relationships of rotifers, comparative studies on mitochondrial genomes of rotifers, studies on cryptic species of rotifers, molecular identification of rotifers, and geographic genealogy of rotifers.