Abstract:In order to investigate the effects of carbonate alkalinity on the growth and ovary development of the ridgetail white prawn Exopalaemon carinicauda. Three carbonate alkalinity gradients of 3 mmol/L (control group), 5 mmol/L and 8 mmol/L were set to culture E. carinicauda for 60 days. The effects of different carbonate alkalinity stress on the growth performance, tissue structure, enzyme activity and ovary development of E. carinicauda were compared. The results showed that there was no significant difference between the 5mmol/l group and the control group in growth gain, weight gain and survival rate (p>0.05), and the growth gain, weight gain and survival rate of the 8 mmol/l group were significantly lower than those of the control group (p<0.05); The tissue sections of hepatopancreas showed that the volume of some B cells increased in 5mmol/l group, and particulate matter appeared in some transport vesicles, and the morphological structure of the lumen was severely deformed in 8mmol/l group. The gill tissue section showed that in the 5 mmol/l group, there was slight swelling of gill filaments, slight deformation of the stratum corneum, expansion and lengthening of the space under the stratum corneum, and disordered arrangement of epithelial cells and pillar cells; In 8 mmol/l group, gill filaments were swollen, arranged irregularly, blood cells swelled, gill filaments epithelial cells were seriously damaged, the structure and morphology of capillary network were changed, and the space under the stratum corneum was deformed. The results of muscle tissue enzyme activity showed that the carbonic anhydrase activity of 5mmol/l group was significantly higher than that of the control group (p<0.05), and the Na+/K+-ATPase activity had no significant difference in muscle (p>0.05); The results of enzyme activity determination in hepatopancreas showed that there was no significant difference in carbonic anhydrase in hepatopancreas (p>0.05), Na+/K+-ATPase activity in 8 mmol/l group was lower than that in other groups (p<0.05), and Na+/K+-ATPase activity in gill tissue in 5 mmol/l and 8 mmol/l groups was significantly higher than that in control group (p<0.05), and the difference between the two groups was significant.; The ovaries of the three groups were able to develop, and the percentages of their development to phase II were 20.51%, 10.52% and 6.25% respectively. There was no significant difference between the 5 mmol/l group and the control group (p>0.05), and there was significant difference between the 8 mmol/l group and the control group (p<0.05); Both 3 mmol/l group and 5 mmol/l group had E. carinicauda with ovary development to stage III, but there was no significant difference between the two groups (p>0.05), and no E. carinicauda with ovary development to stage III was found in 8 mmol/l group.; Ovary tissue sections showed that the arrangement of oocytes in 8 mmol/l group was loose, and the arrangement of surrounding follicular cells was loose and the number was small. To sum up, E. carinicauda can grow and develop in a carbonate alkalinity of less than 8 mmol / L, but high carbonate alkalinity may cause damage to the gills and hepatopancreas of E. carinicauda, affecting its growth. At the same time, it is speculated that high carbonate alkalinity may also affect the development speed of ovaries.