In order to minimize physiological stress, thus improving live P. yessoensis condition and survival rate, commercial hand-dived P. yessoensis, were tied-up during land base relaying, and the effects of stress reduction on storage stability were investigated. One-week supply chain from harvest to market for live scallops was designed including 2-day on-land revitalization (i.e., relaying)and subsequent 4-day chilled dry-storage. Live P. yessoensis were tied-up individually to enforced shell-valve closure pre-relaying as treatment T^b and post-relaying as treatment T^a, changes of live P. yessoensis fitness were investigated compared to the treatment-free control group named C. ATP related substances, nucleotide energy charge AEC, arginine phosphate ArP, pH and glycogen were selected as fitness indicators. Sensory assessment and visual vitality indicated by mantle retraction and mantle response were conducted as well.Results showed that both T^b and T^a had a significant effect on sustaining P. yessoensis fitness in subsequent steps in the supply chain. ① The levels of ATP, ArP, glycogen and other biochemical indicators were in this order: T^b>T^a>C. On the 6^th day, both T^b and T^a presented better fitness than the control C. ② Tying-up held a delayed advanced effect on sustaining fitness in the subsequent dry storage. Results showed that, the order of positive effects was T^b>T^a>C, and a positive correlation between mantle retraction and response time was observed. ③ Sensory analysis results also revealed the advantages of delayed effect induced by tying-up in advance. At the relaying stage, tying-up made no difference in flavor profiles between the groups. Sensory quality of all P. yessoensis tended to decline based on the sensory evaluation. However, it was observed that T^b had always shown obvious advantages in umami and sweetness than others. In summary, the tied-up closure treatment can effectively suppress the physiological stress accompanied by reduced energy consumption. Thereby, high quality live P. yessoensis can be supplied to the market. Based on the experimental results of this study, several potential improvements to current post-harvest handling and transportation of P. yessoensis are suggested.