Environmental endocrine disrupting chemicals(EDCs) can affect the normal endocrine system of organism.Its huge threat to the environment and the health of human beings arouses international attention,which has been the hot topic in environmental research.Vitellogenin(Vtg) production in male and juvenile fish can provide a sensitive biomarker of reproductive disruption to indicate and estimate estrogenicity of EDCs.It has been proved that the vitellogenin of Tanichthys albonubes is a perfect biomarker to be used to monitor EDCs in environment.The objective of the present study was to detect the estrogenic responses of estradiol-17 beta(E₂),nonylphenol(NP),polychlorinated biphenyls(PCBs),Cd²⁺,Zn²⁺ and its mixture in T.albonubes.Native-polyacrylamide gel electrophoresis(Native-PAGE),lipo-staining method,indirect enzyme-linked immunosorbent assay(ELISA) were used to analyze the whole body homogenate from treated male T.albonubes.The results of Native-PAGE showed that after exposure to 10 μg/L E₂,50 μg/L E₂ for 7 days or 14 days,the male T.albonubes could synthesize Vtg.It also showed that the more time,the more vitellogenin and the higher the concentration of E₂,the more Vtg.The results of ELISA showed that the male T.albonubes could be induced to synthesize Vtg after exposure to 0.1,0.5,1.0 μg/L E₂ for 7,14 days or exposure to 200,300 μg/L NP for 14 days.The combined effects of NP,PCB,Cd²⁺ or Zn²⁺together with different level E₂ were dissimilar with their individual effects.The interaction of NP and E₂ was synergism action.This article also discussed the combined effects of environmental estrogen mechanism.