A fibroblast-Like cell line derived from kidney of healthy Grass Carp (Otenoaolaayragodon 2deLtus) was esablisbed and designated CIK which has been passed for126 time since its initiation in January, 1982. The medium used in the cultivation is Eagle MEM (Nissui eiyaku Co LTD)supplemented with 10-15% fetal bovine serum. The incubation temperature is at 28℃. Chromosome number at passage level of 88 ranked from 28 to 117 with a rxiodalnumber of 55.CIK cell line is susceptible to FRV.