夏季北黄海贝类养殖区环境因子、长牡蛎糖原和免疫指标变化的调查研究
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大连海洋大学

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S944

基金项目:

国家自然科学基金(41961124009)


Investigation on the variation of environmental factors, glycogen and immune parameters of Pacific oyster (Crassostrea gigas) in the North Yellow Sea shellfish farming area in summer
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Dalian Ocean University

Fund Project:

the International Cooperation Projects of the National Natural Science Foundation of China (41961124009)

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    摘要:

    为解析夏季环境胁迫和长牡蛎(Crassostrea gigas)机体响应特征,实验针对2021年北黄海夏季高温降雨期的长牡蛎养殖海区开展了2次调查研究和对比分析。结果显示,7月份海区表层水温分别为25.3 ℃和24.9 ℃,盐度分别为26.41和27.87;表层海水中硅藻丰度分别为3.6×103和3×103 个/L,低于往年平均水平,甲藻多样性增加,表层海水中细菌的总丰度分别为2.10×109和2.77×1010 拷贝/L,弧菌丰度分别为3.37×108和5.40×108 拷贝/L;长牡蛎肝胰腺糖原含量分别为40.96和31.58 mg/g、闭壳肌糖原含量分别为6.63和8.91 mg/g;鳃MDA含量分别为13.74和8.69 nmol/mg prot、鳃T-AOC分别为0.46和0.77 U/mg prot;海区养殖长牡蛎血淋巴细胞中CgIL17-5和CgTNF-1的mRNA表达量在第一次调查时相对室内暂养长牡蛎均剧烈升高;血淋巴细胞中CgCaspase3的mRNA表达量升高但差异不显著。综上,2021年北黄海贝类养殖区在经历6—7月夏季高温降雨期后出现水温升高和盐度降低现象,硅藻丰度较往年平均水平下降而甲藻多样性增加,细菌总丰度较入夏前水平降低而弧菌丰度显著升高。与室内暂养对照组长牡蛎相比,海区养殖长牡蛎的糖原含量下降,并发生轻度氧化应激。研究结果有助于我们进一步理解夏季养殖环境的变化规律和长牡蛎机体响应特征,为预防夏季大规模死亡发生提供理论依据和参考。

    Abstract:

    Summer mass mortality is one of the major bottlenecks limiting the green and high-quality development of the Pacific oyster Crassostrea gigas aquaculture in China. The frequent environmental stresses in summer are important causes of mass mortality. In this study, two surveys were conducted for C. gigas aquaculture in the North Yellow Sea during the summer heat and rainfall period. It was found that, for the first and second survey in July, the surface water temperature was 25.3 and 24.9 ℃, respectively. The salinity of the two surveys was 26.41 and 27.87, respectively. The abundance of diatoms of the two surveys was 3.6×103 and 3×103 cells/L, respectively, which was lower than the average level of previous reports, and the diversity of dinoflagellates increased. For the two surveys, the abundance of total bacteria in the water was 2.10×109 and 2.77×1010 copies/L, respectively, and the abundance of Vibrio was 3.37×108 and 5.40×108 copies/L, respectively. The glycogen content of hepatopancreas of the two surveys decreased to 40.96 and 31.58 mg/g, respectively, and the glycogen content of adductor muscle decreased to 6.63 and 8.91 mg/g, respectively. MDA content in gill of the two surveys was 13.74 and 8.69 nmol/mg prot, respectively, and T-AOC in gill was 0.46 and 0.77 U/mg prot, respectively. The expression levels of CgIL17-5 and CgTNF-1 mRNA in haemocytes dramatically increased in the first survey compared to the oysters cultured indoor. The expression levels of CgCaspase3 mRNA in haemocytes increased compared to the oysters cultured indoor, but the differences were not significant. In conclusion, the results showed higher water temperature and lower salinity happened in shellfish farming areas in summer. The abundance of diatoms decreased compared to the average level of previous reports, and the diversity of dinoflagellates, the potentially harmful algae, increased. The abundance of environmental bacteria changed during the environmental stresses, and the risk of outbreak of potential pathogenic bacteria Vibrio increased. Organism responses were observed in oysters including: lower glycogen content and mild oxidative stress. These results helped us to further understand the environmental patterns and response characteristics of the organism during summer and provided a theoretical basis and reference for the prevention and control of the oyster mass mortality in summer.

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  • 收稿日期:2022-03-05
  • 最后修改日期:2022-06-19
  • 录用日期:2022-12-11
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