3种黄颡鱼SOCSs基因分子特性及致病菌胁迫下的表达模式
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Q 785;S 917.4

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浙江省淡水水产研究所开放课题 (ZJK202003);江苏省农业科技自主创新资金[CX(19)2034];江苏省农业重大新品种创制项目 (PZCZ201742);江苏省重点研发计划 (BE2017377)


Molecular characteristics and expression pattern analysis under pathogen stress of SOCSs genes in three species of yellow catfish
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The Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural affairs; Key Laboratory of Freshwater Aquaculture genetic and breeding of Zhejiang Province; Zhejiang Institute of Freshwater Fisheries (ZJK202003), Jiangsu Agriculture Science and Technology Innovation Fund[CX(19)2034], The Creation Project of Major New Species of Agriculture in Jiangsu Province (PZCZ201742), The Key Research and Development Program of Jiangsu Province (BE2017377).

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    摘要:

    细胞因子信号传导抑制因子(SOCS)是JAK/STAT信号通路中细胞因子信号受体的重要抑制因子。为探究SOCS在鱼类免疫应答过程中的作用,本研究以杂交黄颡鱼“黄优1号”及其母本黄颡鱼和父本瓦氏黄颡鱼为对象,克隆了杂交黄颡鱼“黄优1号” 3个免疫相关基因(SOCS1、SOCS2和SOCS3),并对其生物信息学进行了比较分析;采用qRT-PCR技术研究了SOCS1、SOCS2和SOCS3基因分别在3种黄颡鱼感染嗜水气单胞菌和鮰爱德华氏菌后的表达模式。结果显示,杂交黄颡鱼“黄优1号” SOCS1、SOCS2和SOCS3基因的全长分别为561、735和672 bp,分别编码186、244和223个氨基酸,其预测的蛋白结构均含有保守的SH2结构域和SOCS盒。黄颡鱼、瓦氏黄颡鱼和杂交黄颡鱼“黄优1号” SOCS1、SOCS2和SOCS3基因的表达在被检的8个不同组织中呈现组织特异性。在两种致病菌感染后,杂交黄颡鱼“黄优1号”及其双亲的SOCS1、SOCS2和SOCS3基因在肝脏、鳃和头肾组织中的表达量均在前24 h显著升高,随后逐渐恢复至正常水平。此外,鮰爱德华氏菌感染后,杂交黄颡鱼“黄优1号” SOCS1、SOCS2和SOCS3基因的表达量显著高于双亲,表现出较双亲更强的免疫调节能力且具有病原特异性。该结果为进一步解析黄颡鱼SOCS家族免疫防御调控机制提供了参考依据。

    Abstract:

    Suppressor of cytokine signaling (SOCS) is a class of intracellular proteins that inhibited cytokine signaling in JAK/STAT pathway. To explore the function of SOCS in fish immune response, hybrid yellow catfish “Huangyou-1” and its female parent Pelteobagrus fulvidraco and male parent P. vachell were selected as the research object in this study. The immune related genes SOCS1, SOCS2 and SOCS3 of hybrid yellow catfish “Huangyou-1” were cloned and preliminarily analyzed by bioinformatics. The Quantitative Real-time PCR (qRT-PCR) technology was used to detect the expression patterns of these three genes after infection of Aeromonas hydrophila and Edwardsiella ictaluri into hybrid yellow catfish “Huangyou-1” and its parents. The results show that the ORF length of SOCS1, SOCS2 and SOCS3 genes of hybrid yellow catfish “Huangyou-1” was 561, 735 and 672 bp, encoding 186, 244 and 223 amino acids respectively, and prediction results revealed that SOCS1, SOCS2 and SOCS3 proteins contained SH2 conserved domains and SOCS box. The expression pattern of SOCS1, SOCS2 and SOCS3 genes showed tissue specificity in hybrid yellow catfish “Huangyou-1”, P. fulvidraco and P. vachell. After being challenged with A. hydrophila or E. ictaluri, the transcripts of SOCS1, SOCS2 and SOCS3 were all significantly increased, reached the peak at 24 h and then gradually returned to normal levels in liver, gill and head kidney in hybrid yellow catfish “Huangyou-1” and its parents (P<0.05). In addition, compared with parent P. fulvidraco and P. vachell, hybrid yellow catfish “Huangyou-1” had higher mRNA levels in SOCS1, SOCS2 and SOCS3 after the infection of E. ictalurid, showing a stronger immune regulation ability and having pathogen specificity. Our study provides a basis for the analysis of the immune defense regulation mechanism of the SOCS family of Pelteobagrus.

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孙亦如,张红燕,毕宜慧,马思思,张凯,暨杰,王涛,贾永义,尹绍武.3种黄颡鱼SOCSs基因分子特性及致病菌胁迫下的表达模式[J].水产学报,2022,46(9):1537~1552

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  • 收稿日期:2020-10-29
  • 最后修改日期:2021-03-05
  • 录用日期:2021-04-11
  • 在线发布日期: 2022-09-01
  • 出版日期: