鳗鲡疱疹病毒对欧洲鳗鲡的致病性
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S941.41

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福建省公益类科研院所基本科研专项(2018R1019-5);国家自然科学基金(31101933);福建省农业科学院项目(A2016-3,AC2017-1)


Pathogenicity of Anguillid herpesvirus to Anguilla anguilla
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    摘要:

    为了明确鳗鲡疱疹病毒(Anguillid herpesvirus, AngHV)的致病性,本实验采用一株从鳗鲡“脱黏败血综合征”病料中分离的AngHV(NA16108),研究了其对欧洲鳗鲡幼鳗的致病性。结果显示,注射AngHV的鳗鲡体表出现黏液脱落、鳍条出血、红头等症状;鳃部出现黏液增多、出血,肝脏褪色、肿大,脾脏和肾脏肿大等病变;进一步的组织病理学观察发现,鳗鲡的体表黏液及黏膜上皮细胞脱落,次级鳃瓣增生、呼吸细胞肿胀坏死,脾脏细胞坏死、黑色素细胞聚集,肾小管管壁上皮细胞坏死、管腔变窄等病理症状;这与鳗鲡“脱黏败血综合征”的发病特征一致。致死率分析显示,从第4天开始攻毒组鳗鲡开始出现死亡,第7天和第14天的累计死亡率分别达到26.7%和56.7%;荧光定量PCR检测显示,在攻毒鳗鲡肝脏、脾脏、肾脏、肠道、鳃和皮肤黏液中均可检测到AngHV;另外,用鳗鲡卵巢细胞系(eel ovary cell line, EO)从攻毒鳗鲡主要内脏器官中重新分离出AngHV,表明鳗鲡发生了AngHV的系统侵染。研究表明,AngHV是鳗鲡“脱黏败血综合征”的致病病原,这为深入开展该病的发病机制及防控研究奠定了基础。

    Abstract:

    Anguillid herpesvirus (AngHV) is an important viral pathogen of cultured Anguilla anguilla, and also an important factor in the decline of wild A. anguilla populations. Since the 1990s, A. anguilla culture industry has developed rapidly in China, accompanied by frequent outbreaks of an eel epidemic disease with symptoms of “skin mucus sloughing, red head, and septicemia”, which was named eel “mucus sloughing and hemorrhagic septicemia disease”. The disease is the most common and harmful infectious disease of the cultured juvenile A. anguilla, and occurs every year in the eel farms in Fujian Province, causing huge economic losses to the breeders. We detected and isolated AngHV from the diseased A. anguilla samples. In order to determine the pathogenicity of AngHV, an AngHV strain (NA16108) isolated from “mucus sloughing and hemorrhagic septicemia disease” samples was used to study its pathogenesis to juvenile Anguilla anguilla. 15-20 g body weight A. anguilla were intraperitoneally injected with 106 PFU of NA16108, and clinical symptoms, histopathological changes, viral replications in the major organs and tissues, etc, were used to evaluate its pathogenicity. The results showed that the body surface of the AngHV-injected A. anguilla had the symptoms of mucus exfoliation, fin hemorrhages, red head, etc. The diseased A. anguilla had pathological changes of mucus increasing and hemorrhages of gill, discoloration of liver and swelling of spleen and kidney, etc; further histopathological analysis showed that the skin had exfoliation of mucus and mucosa flat epithelial cells; gill had secondary lamellar hyperplasia, blood vessel hemorrhages, swelling and necrosis of respiratory cells; spleen had necrosis and aggregation of melanocytes, and kidney had necrosis wall cells and narrowing lumen of renal tubular, which are consistent with the clinical characteristics of A. anguilla "mucus sloughing and hemorrhagic septicemia disease". The mortality analysis showed that AngHV infected A. anguilla began to die from the 4th day post injection, and the cumulative mortality on the 7th and 14th day reached up to 26.7% and 56.7%, respectively. However, the eels in the control group had no obvious pathological symptoms during the experimental period. Quantitative real-time PCR analysis showed that AngHV could be detected in the liver, spleen, kidney, intestine, gill and skin mucus of AngHV infected A. anguilla with higher copy number in the gill and skin mucus, and AngHV was not detected in the corresponding tissues of the control group. Viral isolation experiment showed that AngHV could be re-isolated by EO cell line from the main internal organs of the injected A. anguilla, indicating that the injected A. anguilla had a systemic infection of AngHV. In addition, we found that the quantity of Pseudodactylogyrus in the challenged group increased rapidly, reaching to about 200 and 800 per A. anguilla on the 7th and 14th day post viral injection, respectively, however, the quantity of Pseudodactylogyrus in the control group did not change significantly, and remained within 100 per A. anguilla. The results indicated that AngHV is the pathogenic agent of "mucus sloughing and hemorrhagic septicemia disease", which laid foundation for further study on the pathogenesis and prevention and control of the disease.

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陈强,李英英,杨金先,宋铁英,葛均青.鳗鲡疱疹病毒对欧洲鳗鲡的致病性[J].水产学报,2021,45(6):940~947

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  • 收稿日期:2020-08-09
  • 最后修改日期:2020-09-16
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  • 在线发布日期: 2021-06-16
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