快速获得大量鱼类肾细胞中期分裂相的PHA体内注射法
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A PHA INJECTION METHOD IN VIVO FOR THE RAPID OBTAINMENT OF LARGE NUMBERS OF METAPHASE FIGURES FROM KIDENY CELLS OF TELEOSTS
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    摘要:

    本文报导了PHA(Phytohemagglutinin 植物血球凝集素)体内注射制备鱼类肾细胞染色体的方法。在北京鳊、广东鲂、鲮鱼、倒刺鲃.胡子鲶、鳗鲡、乌鳢、罗非鱼等分属不同科、目的近♀0 种鱼类均获得较高的分裂指数。PHA 体内注射法的平均细胞有丝分裂指数为肾培养的1.56倍,血培养的1.82倍,常规空气干燥法的4.51倍,达到9.86%。研究结果表明,PHA 在体内同样具有刺激肾细胞有丝分裂的作用。PHA 注射剂量8—10μg/g(鱼体重),秋水仙素注射剂量4—6μg/g(鱼体重),胸鳍基部各一次注射,效应时间4—4.5小时,在7小时内便可获得大量中期分裂相。所建立的方法不需进行细胞体外培养,简便快捷,适用于实验室和野外现场制备鱼类染色体。

    Abstract:

    The method of kideny cell-PHA (phytohemagglutinin)culture in vitro for thestudy of fish chromosomes was reported by Yamamoto, K. and Y. Ojima (1973). Inthis paper a new method for inducing fish chromosomes divisions is proposed. The PHAadministration was by pericardial cavity injection in vivo, instead of adding it into thecell culture medium in vitro. The injection does of PHA is 8-10μg/g (body weight),the injection position is better located at the base of pectoral fin, the time for responseneeds about 4-4.5 hours after injection. It needs only 7 hours to obtain a largenumbers of mitotic metaphase figures of kidney cell. About 30 species of teleost fisheswere examined in the experiments. e. g. Parabramis pekinensis, Megalobrama hof-fmanni, Barbodes denticulatus, Cirrhinus molitorella, Clarias fuscus, Monopterusalbus, Anguilla japonica, Ophicephalus argus, Tilapia mossambica etc. all givingsatisfactory results. The average percentage of mitotic division by PHA injectionmethod is 1.56 times than that of the kidney cell-PHA culture; 1.82 times of theblood-PHA culture and 4.51 times of the usual air-drying, reaching 9.86%. Thismethod has a double advantage being rapid and simple, and also adaptable to thefield work.

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林义浩.快速获得大量鱼类肾细胞中期分裂相的PHA体内注射法[J].水产学报,1982,6(3):201~208

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  • 在线发布日期: 2014-10-27
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